Anti-inflammatory and Xanthine Oxidase Inhibition Activities of Santolina chamaecyparissus Extracts

S. chamaecyparissus aerial parts were extracted with solvent of varying polarity: crude extract (CrE) chloroform extract (CHE), ethyl acetate extract (EAE), and aqueous extract (AE). The content of total phenolics, and flavonoids in all the extracts were determined with spectrophotometric methods. Among all the extracts analyzed, the EAE exhibited a higher phenolic and flavonoids content than other samples: 373.83 ± 0.23 mg gallic acid equivalent and 7.86 ± 61.51 mg quercetin equivalent/g of dried weigh, respectively. CHE and EAE, showed a high inhibition of xanthine oxidase (XO) P>0.05 with IC50(concentration inhibitory of 50% of XO activity): (0.051± 0.0002 mg/ml) and (0.052±0.0003 mg/ml) respectively, followed by CrE (0.091±0.001 mg/ml). The inhibition of xanthine oxidase by CHE and EAE showed a less efficient than allopurinol (IC50 = 0.0082 ± 0.0005 mg/ml). The CrE clearly demonstrated anti-inflammatory effects by reduced ear edema induced by PMA with 61.51%.