Biochemical analysis between In Vivo and In Vitro Regenerated Plant Leaves of Solanum nigrum L.

To compare the efficacy for biochemical analysis of in vitro propagated plants and field grown plants of Solanum nigrumL. Maximum shoot multiplication were observed MS medium containing 0.75 mg/l BAP+0.5 mg/l IBA and 0.1 mg/l NAA. Immature tissues showed better response for culture studies. The biochemical analysis (Starch, Sugar, Protein and Amino acids) from in vitro and in vivo plant leaves of S. nigrum L. Starch content was 121.91 mg/g fresh weight in in vivoplant leaves but it was 60.14 mg/g in in vitro plant leaves. Sugar content was 201. 86 mg/g fresh weight in in vivo plant leaves but it was 106.13mg/g fresh weight in vitro plant leaves. Protein content was 191.12 mg/g fresh weight in in vivoplant leaves but it was 96.73 mg/g fresh weight in in vitro plant leaves. The Amino acid content was also high in in vivoplant leaves 238.21 mg/g fresh weight in in vivo plant leaves but it was 116.15 mg/g fresh weight in in vitro plant leaves. The biochemical content was more in in vivo plant leaves than the in vitro plant leaves. Further studies may be carried out to improve the biochemical substances in in vitro plants.