EFFECTS OF SALINITY AND EXOGENOUS PROLINE APPLICATION ON PROTEIN PROFILING AND ANTIOXIDANT ENZYME ACTIVITIES DURING Ex vitro SHOOT MULTIPLICATION IN HYPOCOTYLS OF TWO BRUGUIERA SPECIES

Healthy and mature propagules/hypocotyls of two Bruguiera species i.e Bruguiera gymnorrhiza and Bruguiera parviflora were collected from Bhitarakanika mangrove forest (Buffer zone), Odisha, India and raised in green house condition. The propagules were decapitated/excised in the collar region (meristematic junction between plumule and hypocotyls). The decapitated hypocotyls were treated with the different concentration of salt and/or proline medium (T1 to T7) and control (T0) at 10 days intervals up to 40 days. Samples (hypocotylar part) were collected at 10 days intervals up to 40 days and analyzed in order to study the changes with effect of different concentration of NaCl and/or proline on expression of salt induced protein markers and antioxidant enzyme activity during multiple shoot regeneration in two Bruguiera species. Total protein content decreased with increasing concentration of salt stress but after amendment of proline, it was gradually increased in both the species. B. gymnorrhiza synthesized an unique polypeptide of MW 17 KDa with high salinity condition at 30 days of decapitation. Similarly, polypeptides having MW 41, 47 and 32 KDa synthesized due to high salinity after 40 days in case of B. parviflora. Likewise, the antioxidant enzymes such as POX, CAT and SOD were accumulated more in B. gymnorrhiza than B. parviflora. Hence, it is concluded that sodium chloride stress tolerant potential was more in B. gymnorrhiza than B. parviflora. The findings may contribute to better multiple shoot from hypocotyl to raise a salt tolerant line for mangrove restoration purpose.