MORPHOLOGICAL CHARACTERISATION OF ZINC SULFIDE NANOPARTICCLES USING ELECTRON MICROSCOPY AND X-RAY DIFFARACTION ASSAY

Semiconductor nanoparticles are having a wide range of applications in biomedical imaging and therapeutics. In this present investigation, we used marine bacteria Enterococcus sp. RMAA for the biosynthesis of zinc sulfide nanoparticles (ZnS NPs) by extracellular route is a low cost and eco-friendly manner. The synthesised ZnS NPs were characterised using different techniques such as UV-vis spectrophotometer, X-ray diffraction assay (XRD), scanning electron microscope (SEM), elemental dispersive analysis of X-rays (EDX) and transmission electron microscopy (TEM). XRD result confirms the crystalline nature of ZnS nanoparticles identified by reflection planes. The clumped, as well as polydispersed particles with biological compounds, are observed in the microscopic analysis (SEM and TEM). The EDX spectrum shows the presence of absorption peak of Zn and S in the biosynthesised nanoparticles with some minor peaks represents from biomolecules of culture supernatant. Finally, the ZnS NPs were tested against different pathogens isolated from clinical samples. In that Bacillus subtilis and Staphylococcus aureus shows a good zone of inhibition. The research concluded the ZnS nanoparticles would play an essential role in the production of nanomedicine for various epidemic diseases.