INDUCTION OF POTATOES (Solanum tuberosum L.) MICRO TUBER AT SOME COUMARIN CONCENTRATIONS AND INCUBATION TEMPERATURES

Potatoes are a priority horticultural crop to be developed because they can be used for food diversification. Increasing the production of micro tubers is needed for the provision of potato seeds. The purpose of this study was to obtain the interaction between coumarin concentration and incubation room temperature on the induction of potato micro tubers. This research was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Andalas University, Padang from September 2019 to January 2020. The study was a two-factor factorial with 4 replications in a completely randomized design (CRD). The first factor is the concentration of coumarin which consists of 5 levels, namely 0, 25, 50, 75 and 100 mg / l. The second factor is the temperature of the incubation room which consists of 3 levels, namely: 20, 25 and 30 ° C. The data were analyzed by using the F test and followed by the Tukey’s test. The research data showed that there was an interaction between the application of several coumarin concentrations and the incubation room temperature to time of tubers emergence, the fastest tubers appeared 13.26 days after the addition of the tuber medium (DAT) at 100 ppm of coumarin and a temperature of 30 ° C. The best application of 100 ppm coumarin for time of tubers emergence, number of tuber and fresh weight of root. Incubation room temperature affected time of tubers emergence, but did not affect diameter of tuber, fresh weight of tuber and fresh weight of shoot.