Advanced Studies on Anticoagulant Activity and Molecular Docking of 7,9-bis(4-Chlorophenyl)- 6- methyl-1,4-dioxa-8-azaspiro[4.5]decane through Target Protein Thrombin

The asymmetric point of the molecules due to the chiral spiro carbon is one of the significant criterions
for their biological activities. Spiro compounds have potential biological properties. The present study
reported the synthesis and anticoagulant activity of spiro compounds, the potential compound was
subjected to study their binding mode and interaction using bioinformatics tools. Among the five
compounds tested, 7,9-bis(4-chlorophenyl)-6-methyl- 1,4,8-triaza spiro[4.5]decane has prolonged
activated partial thromboplastin time and prothrombin time as 22.7s and 18.5s at 25 μg mL-1,
respectively. Hence, the mandatory way study was carried out for this compound by using
bioinformatics method. Among the five compounds, compound 9 was identified as potent
anticoagulant (25 μg mL-1) against activated partial thromboplastin time and prothrombin time assay.
The interaction results shows that this compound binds in the active site of the target protein thrombin
which is similar to that of existing inhibitor and it may also considered as an anticoagulant drug in
future.